![]() The acidic heteropolysaccharide is a major component in cereals, vegetables, and fruits. Pectin is a structural acidic heteropolysaccharide rich in galacturonic acid with carboxyl groups esterified with the methanol. This enzyme breaks down pectin commonly found in the plant cell wall and, hence, is well-known for the commercial preparation of clear fruit juice, liquefaction and saccharification of plant biomass, paper making, as well as coffee and tea fermentation ( 5). The enzyme pectinase has engrossed interest worldwide as a biological catalyst in various industrial processes. There have been number of microbe-borne enzymes (amylase, cellulase, glucosidases, invertase, keratinases, lactase, ligninase, lipase, penicillinase, protease, xylanase, etc.) developed and commercially popularized due to their highly significant action as well as economic feasibility ( 3, 4). Protein engineering, in addition to recombinant processes, is an approachable alternative to engineer recombinant enzymes that enhance the rate of action in various aspects. On the other hand, the biotechnological approaches involving the identification of microbial enzymes, mechanisms of action, and scaled-up production are a critical challenge to the researchers ( 1, 2). This is because the enzymes could be used as a substitute for some toxic chemicals that were previously involved in food processing. Over the past centuries, there has been a notable increase in the application of enzymes in various sectors of industry. Furthermore, various production methods related to the optimization of the product and its significant contribution to the pharmaceutical industry (either pectinase or derived pectic substances) are described in this article. In the present review, an effort has been made to orchestrate the fundamental information related to structure, depolymerization characteristics, and classification of pectin as well as the types and biochemical properties of pectinase. For instance, gaining knowledge with respect to the versatile molecular heterogeneity of the compounds could be considered as the center of concern to resolve the industrial issues from multiple aspects. ![]() ![]() Understanding pectic substances and their structure, unique depolymerization, and biochemical properties such as a catalytic mechanism and the strong interrelationship among these molecules could immensely enhance their applicability in industries. These molecules are a feasible non-toxic contrivance of nature with extensive applicative perception. Both pectin and pectinase are vitally imperative biomolecules in the biotechnological sector. ![]()
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